Table 1 The primers of LAMP assay and PCR assay for tet(X2), tet(X3), tet(X4), and tet(X5) genes
Gene | Primer | Sequence (5′−3′) | Tm (℃) | size (bp) | Reference |
|---|---|---|---|---|---|
tet(X2/X3/X4/X5) | F3 | GTTGGACTGACTATGGCA | 51 | - | This study |
B3 | ACCCATTGGTAAGGCTAAG | 51 | This study | ||
FIP | CCTCTCGGTCGTTGTCTCTTTAAATTATTACAGCAAAACGGCA | 65 | This study | ||
BIP | CAAGAATTTTTGGTGGAACCCTTGTAAGTTTGTAACAATCCCGCTT | 65 | This study | ||
LB | CAAAGGTTCAGGTCAGGAAGCAATG | 58 | This study | ||
tet(X2) | Forward | CGGGATGTCCAAGGTAAGAAAA | 54 | 434 | [12] |
Reverse | TGACAACGTCGTATGAATCAA | 51 | |||
tet(X3) | Forward | GGTATCAACATTTCAATGCTTG | 49 | 265 | [12] |
Reverse | CGATTCGTCCTGCGTATCTTTTG | 56 | |||
tet(X4) | Forward | CTGATTCGTGTGACATCATCTTTTG | 53 | 204 | [12] |
Reverse | GTTAAATTTCCCATTGGTCAGATTA | 50 | |||
tet(X5) | Forward | TGCCGTTGACCTACACAAAGG | 57 | 161 | [15] |
Reverse | TGTCAAAACGATTTTCGGGTC | 53 |