Skip to main content
Fig. 2 | BMC Microbiology

Fig. 2

From: Sensitive and rapid detection of tet(X2) ~ tet(X5) by loop-mediated isothermal amplification based on visual OTG dye

Fig. 2

1) LAMP assay specificity for tet(X2/X3/X4/X5) among 16 of 48 bacteria without the tet(X2/X3/X4/X5) gene. 2–5) sensitivities of LAMP assay (A and B) and PCR (C and D) for tet(X2/X3/X4/X5). Lane M, Ladder H1-K (100 ~ 3000 bp) DNA marker. In A and C, lane 1–7, serial 10-fold dilutions of bacterial lysates from 106 CFU/mL to 101 CFU/mL. Lane 8, negative (water). In B and D, lane 1–7, serial 10-fold dilutions of plasmid DNA from 2 pg/µL to 0.02 fg/µL. Lane 8, negative (water)

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us