Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria

Duche, Rachael T.; Singh, Anamika; Wandhare, Arundhati Ganesh; Sangwan, Vikas; Sihag, Manvesh Kumar; Nwagu, Tochukwu N. T.; Panwar, Harsh; Ezeogu, Lewis. I.

doi:10.1186/s12866-023-02883-0

Table 2 Primer pairs and PCR conditions used for detection of selected antibiotic resistance coding genes

From: Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria

Determining resistance to	Target gene	Primer sequence (5’→3’)	Amplicon size (bp)	Annealing temperature (°C)	Reference
Tetracyclines	tetM	GTG GAC AAA GGT ACA ACG AG CGG TAA AGT TCG TCA CAC AC	406	60.5	[28]
	tetK	GAT CAA TTG TAG CTT TAG GTG AAG G TTT TGT TGA TTT ACC AGG TAC CAT T	155	60.5
	tetL	TGG TGG AAT GAT AGC CCA TT CAG GAA TGA CAG CAC GCT AA	229	60.5
	tetO	AAC TTA GGC ATT CTG GCT CAC TCC CAC TGT TCC ATA TCG TCA	515	60.5
	tetW	GAG AGC CTG CTA TAT GCC AGC GGG CGT ATC CAC AAT GTT AAC	168	60.5	[29]
Macrolides and lincosamides	ermA	CCC GAA AAA TAC GCA AAA TTT CAT CCC TGT TTA CCC ATT TAT AAA CG	590	60.5	[28]
	ermB	TGG TAT TCC AAA TGC GTA ATG CTG TGG TAT GGC GGG TAA GT	745	60.5
	mefA/E	CAA TAT GGG CAG GGC AAG AAG CTG TTC CAA TGC TAC GC	317	60.5
	ermC	AAT CGT CAA TTC CTG CAT GT TAA TCG TGG AAT ACG GGT TTG	299	60.5	[30]
	lnuA	GGT GGC TGG GGG GTA GAT GTA TTA ACT GG GCT TCT TTT GAA ATA CAT GGT ATT TTT CGA TC	323	60.5	[29]
Aminoglycosides	aac(6’)-Ie-aph(2”)-Ia	CAG AGC CTT GGG AAG ATG AAG CCT CGT GTA ATT CAT GTT CTG GC	348	57	[31]
	aph3IIIa	GGC TAA AAT GAG AAT ATC ACC GG CTT TAA AAA ATC ATA CAG CTC GCG	523	57
	ant(4)-Ia	CAA ACT GCT AAA TCG GTA GAA GCC GGA AAG TTG ACC AGA CAT TAC GAA CT	294	57
	aph(2”)-Ic	CCA CAA TGA TAA TGA CTC AGT TCC C CCA CAG CTT CCG ATA GCA AGA G	444	57
	aph(2”)-Id	GTG GTT TTT ACA GGA ATG CCA TC CCC TCT TCA TAC CAA TCC ATA TAA CC	641	57
	ant(6)-Ia	CGG GAG AAT GGG AGA CTT TG CTG TGG CTC CAC AAT CTG AT	563	57	[32]
	aac(6’)-Ii	TGG CCG GAA GAA TAT GGA GA GCA TTT GGT AAG ACA CCT ACG	410	57	[32]
	aadE	ATG GAA TTA TTC CCA CCT GA TCA AAA CCC CTA TTA AAG CC	1060	51	[33]
Penicillins	blaZ	ACT TCA ACA CCT GCT GCT TTC TAG GTT CAG ATT GGC CCT TAG	240	60.5	[34]
	mecA	AGT TCT GCA GTA CCG GAT TTG C AAA ATC GAT GGT AAA GGT TGG C	533	57	[35]
	int-Tn (Tn916/ Tn1545)	GCG TGA TTG TAT CTC ACT GAC GCT CCT GTT GCT TCT	1028	57	[36]
	bla	CAT ART TCC GAT AAT ASM GCC CGT STT TAA CTA AGT ATS GY	297	51	[34]
Vancomycin	vanE	TGT GGT ATC GGA GCT GCA G GTC GAT TCT CGC TAA TCC	513	51	[29]
Trimethoprim	dfrA	CTT TTC TAC GCA CTA AAT GTA AG CAT TAT CAA TAA TTG TCG CTC AC	474	51	[37]
Trimethoprim	dfrD	GGA AGG GCT TTA CCT GAC AGA AG CGA CAT AAG GCA AGA ACA TAA CAT A	175	51	[37]
Ciprofloxacin	parC	TAT TCY AAA TAY ATC ATT CAR GA GCY TCN GTA TAA CGC ATM GCC G	286	51	[34]

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ISSN: 1471-2180

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