Enhancement of feather degrading keratinase of Streptomyces swerraensis KN23, applying mutagenesis and statistical optimization to improve keratinase activity

Table 5 Analysis of variance (ANOVA) for Response Surface Quadratic Model CCD) by actinomyces mutant SA-27

Source	Sum of Squares	Df	Mean Square	F-value	p-value
Model	7875.76	14	562.55	21.05	< 0.0001	significant
A-Ph	1719.91	1	1719.91	64.35	< 0.0001
B-Inc. Time	307.60	1	307.60	11.51	0.0040
C-Sucrose	83.42	1	83.42	3.12	0.0976
D-Yeast Extract	36.78	1	36.78	1.38	0.2591
AB	2.10	1	2.10	0.0784	0.7833
AC	132.42	1	132.42	4.95	0.0418
AD	18.17	1	18.17	0.6797	0.4226
BC	21.51	1	21.51	0.8046	0.3839
BD	0.0264	1	0.0264	0.0010	0.9753
CD	67.61	1	67.61	2.53	0.1326
A²	1881.02	1	1881.02	70.37	< 0.0001
B²	72.38	1	72.38	2.71	0.1206
C²	2.41	1	2.41	0.0902	0.7681
D²	61.31	1	61.31	2.29	0.1507
Residual	400.93	15	26.73
Lack of Fit	292.12	10	29.21	1.34	0.3924	not significant
Pure Error	108.81	5	21.76
Cor Total	8276.69	29

ISSN: 1471-2180