From: Enhancement of feather degrading keratinase of Streptomyces swerraensis KN23, applying mutagenesis and statistical optimization to improve keratinase activity
Factors
Independent Factor
Unit
Range Level
Minimum (− 1)
Maximum (+ 1)
X1
Incubation time
Hours
24
72
X2
pH
-
5
9
X3
Sucrose (carbon source)
% (w/v)
0.5
2.5
X4
Yeast extract (nitrogen source)