Fig. 2

Genes or pathways affected in Pseudomonas aeruginosa PAO1 in response to S100A12 treatment. The exact mechanism of action of S100A12 is not known, but it causes a broad transcriptional response. The genes of pyoverdine biosynthesis are downregulated in P. aeruginosa PAO1 in response to S100A12 treatment. pvdA, encoding the enzyme L -ornithine N5-oxygenase, induces the initial step in the pyoverdine synthesis pathway, pvdP turns ferribactin into fluorescent pyoverdine, pvdF and pvdN are important genes in pyoverdine biosynthesis. Quorum sensing lasR system related genes are impeded. Metabolic and redox pathways along with virulence factors as well as transporter and membrane proteins are also impeded. Reduced expression of rhamnolipid biosynthesis related genes rhlG, encoding an NADPH dependent ketoacyl reductase and rhlA, rhlG, as well as QS rhlR system rhlR, rhll- are reduced. Extracellular polysaccharides genes pelA, pelF, and pelG as well as biofilm genes pslA, pslG, algD, and alg44 are downregulated. Oxidative stress defense pathway genes, oxyR, ospR, fpvL and katB are downregulated. Expression of genes implicated in phenazine synthesis pathway, phzA1, phzB1, phzA2, and phzB2 are drastically reduced. Expression of genes related to the structural component of type 6 secretion system (T6SS); pldA, icmF1, hcp1, and vrgG1, are also diminished. This figure is based on Mishra P et al. (2022) [98] publication