Skip to main content
Fig. 2 | BMC Microbiology

Fig. 2

From: Two target genes based multiple cross displacement amplification combined with a lateral flow biosensor for the detection of Mycobacterium tuberculosis complex

Fig. 2

Optimization of reaction temperature for MTBC-MCDA primers. Both the IS6110 gene (A1-A8) and mpb64 gene (B1-B8) were detected at different temperatures. The genes were detected for different temperatures (63–70 °C, 1 °C intervals) with template DNA at the level of 100 pg per reaction. The standard MCDA reactions for the detection of M. tuberculosis were monitored by a real-time turbidimeter, the threshold value was 0.1, and a turbidity > 0.1 was set as positive. The M. avium genomic templates were used as a negative control

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us