Fig. 1

Dietary iron supplementation increases luminal iron concentration of the gut without affecting body weight. A Schematic representation of the experimental design starting with mice fed a diet sufficient in iron and antibiotic delivery for one week, followed by either an iron-sufficient (50 mg.kg^-1) or iron-supplemented (500 mg.kg^-1) diet until day 80 (T80). B Body weight (means ± SEM) between iron-sufficient and iron-supplemented groups. C Fecal non-heme iron measured by ferrozine assay at T80 (means ± SD). N = 9 per group. Differences were assessed by multiple t-test corrected for false discovery rate (fdr) (B) and Welch t-test (C)