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Fig. 8 | BMC Microbiology

Fig. 8

From: Candida albicans and Candida glabrata triosephosphate isomerase – a moonlighting protein that can be exposed on the candidal cell surface and bind to human extracellular matrix proteins

Fig. 8

Displacement of C. albicans Tpi1 from ECM proteins by peptides matching Tpi1 fragments. Samples containing 25 µL of biotinylated Tpi1 (300 nM) and 25 µL of the specified peptide solution (30 µg/mL) were added to the microplate wells containing 3 pmoles of immobilized ECM proteins and the microplates were incubated for 1.5 h at 37 °C. After washing off unbound protein, the amounts of bound Tpi1 were determined using the SA-HR/TMB system. The level of binding of the biotinylated Tpi1 without a competitor added was considered as 100 %. Bars on graph represent the mean values of three determinations, with standard deviations. Statistical significance levels were determined using a one-way ANOVA test against the control sample; *p<0.05 and **p<0.01

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BMC Microbiology

ISSN: 1471-2180

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