Fig. 6

PagN and Rck of S. Typhimurium are able to bind to and induce bacterial invasion, leading to a local remodeling of the host actin cytoskeleton. a CHO and Jeg-3 cells were incubated with either PagN- or Rck-coated beads, respectively. After 30 min of contact between cells and coated beads at 37 °C, cells were washed and then stained by immunofluorescence. Horizontal sections of cells obtained with confocal laser scanning microscopy shows actin staining in red and overlay of beads in green and actin. Representative images are shown with an arrow, indicating the site of actin polymerization and typical structural morphologies. b-c Jeg-3 cells were infected with MC1061-psup or MC1061-rck strain for 1 h at 37 °C (MOI 1:10). The percentages of total cell-associated (b) and internalized (c) bacteria were determined as described in the Materials and Methods. Results are mean values ± SD acquired with three independent experiments with two infected wells per experiment. Results were compared using a Mann Whitney test (**p < 0.01)