Fig. 4

Screening of antibacterial activity of the isolates obtained using resistance guided isolation process against a set of test bacteria. Streak-plating technique was used to screen the antibacterial activity. The actinomycetes strains to be tested were inoculated on YEGA plates in a single line vertically and incubated for 4 days at 32 °C. On the 4th days of incubation, the freshly prepared and properly diluted (adjusted to 0.5 McFarland Standard) test bacteria [1. S. brodie; 2. B. cereus; 3. E. coli; 4. Pseudomonas sp; 5. S. aureus (multidrug-resistant) and 6. Proteus sp.] were inoculated horizontally starting close to the actinomycetes growth line to the end of the plates. The plates were then incubated for 18 h at 37 °C and the DOI was measured in mm using a ruler