Efficacy of 16S rRNA variable regions high-resolution melt analysis for bacterial pathogens identification in periprosthetic joint infections

Table 2 Primers targeting the bacterial 16S rRNA gene for HRMA

Region	Primer	Sequence (5′-3′)	Product size (bp)	Ref
V1	V1-F V1-R	GYGGCGNACGGGTGAGTAA TTACCCCACCAACTAGC	170–171	[49]
V3	V3-F V3-R	CCAGACTCCTACGGGAGGCAG CGTATTACCGCGGCTGCTG	204–205
V6	V6-F V6-R	TGGAGCATGTGGTTTAATTCGA AGCTGACGACANCCATGCA	128–131

ISSN: 1471-2180