Fig. 5From: GroEL protein of the Leptospira spp. interacts with host proteins and induces cytokines secretion on macrophagesBinding of recombinant proteins to elastin. The ability of recombinant proteins to bind to insoluble elastin was evaluated by SDS-PAGE. BSA was used as a negative control. The bound (precipitate) and unbound (supernatant) fractions were analyzed by 12% SDS-PAGE. M: molecular mass protein markers. C: control protein. The asterisk (*) correspond to a product of GroEL degradation that was completely absorbed to the elastinBack to article page