Fig. 2
Effect of different doses of five Fno isolates on Galleria mellonella larva survival. Kaplan-Meier plots of G. mellonella larva survival during 264 h at 28 °C after injection of ca. 1 × 108, 5 × 108, 1 × 109 and 5 × 109 CFU/mL of (a) Fno STIR-GUS-F2f7, (b) Fno Austria, (c) Fno PQ1104, (d) Fno Franc-COS1, and (e) Fno Ehime-1, showing dose-dependent reductions in larval survival. Heat-killed (HK) cells (equal dose to group injected with ca. 5 × 109 CFU/mL; killed by 30 min at 90 °C) and sterile culture filtrates (CF) of each Fno isolate were also injected. Actual CFU/mL after plating bacterial suspensions of each Fno isolate on CHAH presented beside each line on the plots. Survival was 100% in the unmanipulated and PBS only control groups for each replicate (data not shown). n = 24