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Fig. 2 | BMC Microbiology

Fig. 2

From: High-resolution multilocus sequence typing for Chlamydia trachomatis: improved results for clinical samples with low amounts of C. trachomatis DNA

Fig. 2

Representative results for typical nested 2 step amplification and melting curve analysis for each of the hctB (CT046), CT058, CT144, CT172 and pbpB (CT682) genes, the left figure shows the melting curve and the right the amplification plot (multicomponent plot). A) typical successful nested 2 step PCR amplification of the ATCC strains: ATCC VR-885, ATCC VR-886, ATCC VR-878 and ATCC VR-901B. B) Typical successful and unsuccessful nested 2 step PCR amplification of clinical samples: G (Good) successful amplification, B (Bad) Unsuccessful amplification and M (Moderate). Unsuccessful amplification of CT058 of sample 298 versus positive control (a), Successful amplification of CT046 of sample 293 versus positive control (b), Unsuccessful amplification of CT172 of sample 230 versus positive control (c), and Moderate amplification of CT682 of sample 362 versus positive control (d)

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BMC Microbiology

ISSN: 1471-2180

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