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Fig. 3 | BMC Microbiology

Fig. 3

From: Differential production and secretion of potentially toxigenic extracellular proteins from hypervirulent Aeromonas hydrophila under biofilm and planktonic culture

Fig. 3

Hemolytic potential of vAh extracellular proteins secreted under biofilm and planktonic growth. The hemolytic ability of vAh secreted proteins was measured using channel catfish erythrocytes as the substrate. 2.5 μg secreted proteins from each culture condition was incubated with 25 μl catfish blood diluted 1:10 in sterile PBS. at 30 °C with shaking. Sterile, deionized water served as positive control and sterile PBS served as a negative control. Lysis was calculated by measuring sample absorbance at 415 nm, and reported as percent positive control. All samples were assayed in triplicate. Statistical analysis consisted of one-way ANOVA followed by Tukey’s multiple comparisons post-test with significance set at p < 0.05

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BMC Microbiology

ISSN: 1471-2180

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