Fig. 3
From: The aceE involves in mycolic acid synthesis and biofilm formation in Mycobacterium smegmatis
The effect of aceE deficiency on pellicle and biofilm formation of mycobacteria. a. aceE-mut is defective in pellicle formation, while the complementary expression of aceE gene in aceE-mut stain can recover the formation of pellicle in 7H9 medium without Tween-80 in standing culture. Each experiment performed in triplicate. b. Quantification of the biofilm formation after crystal violet staining. Mean optical density for five biological replicates per strain ± SD for a representative experiment from 3 experiments is shown. Significant differences were determined by One Way ANOVA and are indicated by *** (P < 0.001). Error bars represent SD