Table 2 PCR primers used in this study
Primer Name | Sequence* |
|---|---|
Construction of deletion mutants | |
0046HindIIIF | GATCTAAAGCTTGCTCAACATTATTAACGGTTC |
0046R | TTCTAACATAATGAATACCTCGTA |
0046Soe | AGGTATTCATTATGTTAGAAGACTAATCTAGATCAAGATTCATCA |
0046SacIR | GATCTAGAGCTCCATACTACTTCTGCGTCTTC |
0864HindIIIF | GATCTAAAGCTTCTGCTGATATTGATGCAGTGAGTGG |
0864R | CGCACCGGCAATAACTATTCCCTTAAT |
0864Soe | AAGGGAATAGTTATTGCCGGTGCGCGTGCAGAATTAACTCAAGGTCGC |
0864SacIR | GATCTAGAGCTCCGTGCACTTGACACAGATCCTG |
1426HindIIIF | GATCTAAAGCTTTATAGATTAATTGACTGCAGCC |
1426R | CGCTGCCATTGAAGTAATTA |
1426Soe | TAATTACTTCAATGGCAGCGAACTAATCTATTAATGAAGAAACTCGT |
1426SacIR | GATCTAGAGCTCCTTATCGTTCATGCCAAGAA |
1539BamHIF | GATCTAGGATCCATCACTTGATCGATCATCTG |
1539R | CTTCATCTGAATATCTCCTCT |
1539Soe | AGAGGAGATATTCAGATGAAGTTGATAAAATAATCTACTACTTTGTGA |
1539SacIR | GATCTAGAGCTCCTCTTAGGTGCAAGCATTAA |
PCR analysis and DNA sequencing of deletion targets | |
0046up | CTATCTGTATGATGCTTCCAC |
0046dw | GTACCTCAATCTGTTGTAATCTC |
0864up | ACAAGCTAGAGGTATGGCTGG |
0864dw | CCACATGAATGGCGTATGGC |
1426up | AAGCCGTTGTATTGAATGATGGTAG |
1426dw | CGCGAATCATCAATTCACGGTA |
1539up | CAGGATAGGGATGCACATGC |
1539dw | CGACGTTGACGTGTTACTGT |