Strain or Plasmid | Genotype or characteristics | Reference |
---|---|---|
Strains | ||
Lactobacillus acidophilus | ||
NCK56 (NCFM) | Human intestinal isolate | [28] |
NCK1909 (Δupp) | NCFM with a 315 bp in-frame deletion within the upp gene (lba0770); background/parent strain for NCFM deletion mutants | [27] |
NCK1910 | NCK1909 harboring the repA helper plasmid pTRK669; host for pORI-based counterselective integration vector | [27] |
NCK2439 (Δlba0864) | NCK1909 with a 1425 bp in-frame deletion within the lba0864 gene | This study |
NCK2441 (Δlba1426) | NCK1909 with a 717 bp in-frame deletion within the lba1426 gene | This study |
NCK2530 (Δlba0046) | NCK1909 with a 342 bp in-frame deletion within the lba0046 gene | This study |
NCK2608 (Δlba1539) | NCK1909 with a 498 bp in-frame deletion within the lba1539gene | This study |
Escherichia coli | ||
EC101 | RepA+ JM101; Kmr; repA from pWV01 integrated in chromosome; cloning host for pORI-based plasmids | [29] |
NCK1391 | E. coli DH10B harboring pTRK669; cloning host for pORI-based plasmids | [27] |
NCK1911 | EC101 host harboring pTRK935 integration vector | [27] |
NCK2438 | EC101 host harboring pTRK1118 recombinant plasmid | This study |
NCK2440 | EC101 host harboring pTRK1119 recombinant plasmid | This study |
NCK2529 | EC101 host harboring pTRK1126 recombinant plasmid | This study |
NCK2607 | DH10B host harboring pTRK1170 recombinant plasmid | This study |
Plasmids | ||
pTRK669 | Ori (pWV01), Cmr, RepA+, thermosensitive | [30] |
pTRK935 | pORI upp-based counterselective integration vector, Emr | [27] |
pTRK1118 | pTRK935 harboring a mutated copy of lba0864 gene cloned into HindIII/SacI site | This study |
pTRK1119 | pTRK935 harboring a mutated copy of lba1426 gene cloned into HindIII/SacI site | This study |
pTRK1126 | pTRK935 harboring a mutated copy of lba0046 gene cloned into HindIII/SacI site | This study |
pTRK1170 | pTRK935 harboring a mutated copy of lba1426 gene cloned into BamHI/SacI site | This study |