Table 3 Primers and PCR conditions for detecting mupirocin, fusidic acid and retapamulin resistance genes in this study

mupA

mupA-F

TATATTATGCGATGGAAGGTTGG

Initial pre-denaturation at 94 °C for 5 min; 30 cycles of denaturation (30 s at 94 °C), annealing (30 s; at 53 °C for mupA, and 55 °C for mupB, Smr, Mrm and Lmr), and extension (50 s at 72 °C), followed by a final extension step of 7 min at 72 °C

457 bp

mupA-R

AATAAAATCAGCTGGAAAGTGTTG

mupB

mupB-F

CTAGAAGTCGATTTTGGAGTAG

674 bp

mupB-R

AGTGTCTAAAATGATAAGACGATC

Smr

Smr-F

ATAAAGGTAAAAAGCCAGTTTATTGGT

200 bp

38

Smr-R

TAATCGCAACATTTGATGGAATTGTC

Mrm

Mrm-F

TCCCAGCAGATATGTATTTAGAAGGT

450 bp

Mrm-R

AACCACTTGGTCAGGTACAATCACA

Lmr

Lmr-F

GTAAATCTTTAGGTAATGTGATTGTAC

690 bp

Lmr-R

TCTTCTTTAACATGTGGTGTATGAGA

fusA

fusA-F

TTTACCCTGAGTGTGTTCT

Initial pre-denaturation at 94 °C for 5 min; 30 cycles of denaturation (30 s at 94 °C), annealing (30 s; at 53 °C for fusB-D and fusE, and 40 °C for fusA), and extension (2 min, at 72 °C for fusA; 50 s, at 72 °C for other genes), followed by a final extension step of 7 min at 72 °C

2250 bp

fusA-R

TACATTTAAGCTCACCTTGT

fusB

fusB-F

TCATATAGATGACGATATTG

496 bp

26

fusB-R

ACAATGAATGCTATCTCGAC

fusC

fusC-F

GATATTGATATCTCGGACTT

128 bp

fusC-R

AGTTGACTTGATGAAGGTAT

fusD

fusD-F

TGCTTATAATTCGGTCAACG

525 bp

fusD-R

TGGTTACATAATGTGCTATC

fusE

fusE-F

CCTAGTGACGTAACAGTAAC

505 bp

fusE-R

CGGCGWACRTATTCACCTTG

rplC

rplC-F

AACCTGATTTAGTTCCGTCTA

Initial pre-denaturation at 94 °C for 5 min; 30 cycles of denaturation (30 s at 94 °C), annealing (30 s; at 50 °C for vgaA and vgaA_LC, 52 °C for vgaB, vagC, vgaE, lsaA-C and lsaE, and 55 °C for rplC, cfr, vgaAv, salA and 23S RNA V), and extension (2 min, at 72 °C for vgaC, vgaE and salA; 50 s, at 72 °C for other genes), followed by a final extension step of 7 min at 72 °C

822 bp

rplC-R

GTTGACGCTTTAATGGGCTTA

cfr

cfr-F

GAGATAACAGATCAAGTTTTA

1050 bp

39

cfr-R

CGAGTATATTCATTACCTCAT

vgaA

vgaA-F

TCACATGATCGCGCTTTTTTAGAT

770 bp

29

vgaA-R

TCGCTCTCCACCACTTAAGACACT

vgaAv

vgaAv-F

CTCTTTGTACGAGTATATGG

770 bp

40

vgaAv-R

GTTTCTTAGTAGCTCGTTGAGC

vgaA_LC

vgaA_LC-F

CATTATCGCCATCTGTCA

541 bp

9

vgaA_LC-R

AATTCTTCCGAAGGTTCA

vgaB

vgaB-F

TGACAATATGAGTGGTGGTG

577 bp

vgaB-R

GCGACCATGAAATTGCTCTC

vgaC

vgaC-F

CGTATGCCCAGAGTGAG

1073 bp

vgaC-R

GAGTGCTTCCGTATCCA

vgaE

vgaE-F

GAAATATGGGAAATAGAAGATGG

995 bp

vgaE-R

TGATTCTCTAACCACTCTTC

lsaA

lsaA-F

ACCGTGAAGGTGATAAGT

500 bp

lsaA-R

TTGGGTGTAATCTAACTGAT

lsaB

lsaB-F

TCCACTGCCGTTCTTTCC

715 bp

lsaB-R

AGCCATGTACCGTCCTTT

lsaC

lsaC-F

GGCTATGTAAAACCTGTATTTG

429 bp

lsaC-R

ACTGACAATTTTTCTTCCGT

lsaE

lsaE-F

TTGTACGGAATGTATGG

675 bp

lsaE-R

TTCGCTTCTATTAAGCACTCTT

salA

salA-F

CGATGAACCAACAAACCACA

981 bp

10

salA-R

AGGACCGAACCTTGAAATGA

 23S RNA V

23S RNA-F

TGGGCACTGTCTCAACGA

634 bp

41

23S RNA-R

GGATAGGGACCGAACTGTCTC

MLST typing

arcC

arcC-F

TTGATTCACCAGCGCGTATTGTC

Initial pre-denaturation at 94 °C for 5 min; 30 cycles of denaturation (30 s at 94 °C), annealing (30 s; at 55 °C for 7 house-keeping genes), and extension (50 s, at 72 °C for other genes), followed by a final extension step of 7 min at 72 °C

456 bp

arcC-R

AGGTATCTGCTTCAATCAGCG

aroE

aroE-F

ATCGGAAATCCTATTTCACATTC

456 bp

aroE-R

GGTGTTGTATTAATAACGATATC

glpF

glpF-F

CTAGGAACTGCAATCTTAATCC

465 bp

glpF-R

TGGTAAAATCGCATGTCCAATTC

gmk

gmk-F

ATCGTTTTATCGGGACCATC

429 bp

gmk-R

TCATTAACTACAACGTAATCGTA

pta

pta-F

GTTAAAATCGTATTACCTGAAGG

474 bp

pta-R

GACCCTTTTGTTGAAAAGCTTAA

tpi

tpi-F

TCGTTCATTCTGAACGTCGTGAA

402 bp

tpi-R

TTTGCACCTTCTAACAATTGTAC

yqiL

yqiL-F

CAGCATACAGGACACCTATTGGC

516 bp

yqiL-R

CGTTGAGGAATCGATACTGGAAC