Fig. 6
Cytokines secreted by macrophages infection. H37Rv, Δ0148 and CΔ0148 strains after post-infection cytokines were measured according to protocol. The capture antibody was coated onto the wells; to samples of 100 μL were added detection antibody followed by substrate solution to identify the specific cytokines. IL-1β, IL-6 and TNF-α. Two-way ANOVA was performed. The values were taken by considering the average of three triplicate independent experiments. Data represents mean, Standard error mean deviation (SEM) in each experiment and error bars indicate mean ± standard deviation SD. *** significant at P < 0.001 and ** significant at P < 0.01