Role of membrane compartment occupied by Can1 (MCC) and eisosome subdomains in plant pathogenicity of the necrotrophic fungus Alternaria brassicicola

Table 2 Susceptibility of A. brassicicola wild-type and MCC/eisosome mutant strains to different stress conditions

Applied stress	WT	∆abpil1a	∆abpil1b	∆∆abpil1a-abpil1b	∆abnce102	∆abslm1
2 M Sorbitol	39.7 ± 1.9	40.8 ± 2.3	44.9 ± 2	51.5 ± 2.2 *	40.3 ± 1.4	45.3 ± 2.9
100 μM Brassinine	40.4 ± 3	39.8 ± 8	38.7 ± 1.6	36.7 ± 1.9	31.1 ± 4.6	33.3 ± 1.5
60 μM Camalexin	66.3 ± 2.8	58.1 ± 1.3	64.9 ± 3.9	61.8 ± 6.4	53.9 ± 1.5	62 ± 1.8
DMSO (1%)	16.5 ± 1.8	19.7 ± 1.36 *	13.9 ± 4.2	27.4 ± 5.2 *	22.1 ± 1.9 *	10.2 ± 5.1
30 mM Menadione	61.6 ± 3.6	61.1 ± 7	62.6 ± 6.8	59.5 ± 2.8	56.3 ± 10	60.7 ± 2.8
400 mg.L⁻¹ Calcofluor White	55.3 ± 0.9	52.9 ± 1.5	53.4 ± 1.7	57 ± 4.1	50.3 ± 1.7	55.3 ± 1.8
100 mg.L⁻¹ Congo Red	45.7 ± 1.6	42.1 ± 0.9	40.8 ± 5.2	44.9 ± 1.7	40.4 ± 1.1	39.9 ± 4.7

The results are expressed as the percentage of inhibition in treated samples compared to the control without additive. Conidia of each genotype were used to inoculate microplate wells containing standard PDB medium supplemented with the appropriate compound. Nephelometric growth was automatically recorded for 33 h at 24 °C. Each condition was tested in triplicate and the experiments were repeated three times. The areas under the curves were used to calculate the percentages of inhibition for each treatment compared to the control growth curves. Values are means and standard errors of at least nine biological repetitions and represent the percentage growth inhibition under stress conditions compared with standard growth conditions. Conditions denoted with * (p ≤ 0.05) were significantly different compared with their respective control

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