Fig. 5
The effects of Blp1 C-terminal fragment as an immunization agent. a – proliferation rates of LL/2 cells were determined by MTS assay after 24 h of incubation with different concentrations of recombinant Blp1 fragment; error bars represent standard deviations from three repeats; b – titers of Blp1-specific IgG in mice were detected by ELISA (n = 5 mice per group); naïve sera obtained from mice, that received adjuvant with PBS were used as a control; horizontal line represents detection limit of the assay. c – mice survival rates after active vaccination using A. baumannii sepsis model; mice (n = 5 mice per group) were challenged intraperitoneally with 108 CFUs of AbIC I and were monitored for 7 days; d – passive vaccination effect on the mice survival using A. baumannii sepsis model; control group received the serum obtained from mice, immunized with PBS and Freund’s adjuvant; mice (n = 3 mice per group) were challenged intraperitoneally with 5 × 107 CFU of AbIC I and were monitored for 45 days