Fig. 9
Growth and pH assessments of L. crescens cultures in BM-7 medium using four biological buffers. These buffers include Monosodium phosphate (NaP), ACES, Bis-Tris HCl (Bis), and MES at 10, 25 and 50 mM concentrations. L. crescens was grown to exponential phase in Hi-GI at 250 rpm, 28 °C (OD600~0.7–0.8, Fig. 16) and washed with a sterile ACES-KOH buffer pH 6.5 prior to inoculation in BM-7 with the different buffers at an initial pH of 6.5. The growth of L. crescens is similar in all media, except for phosphate at 50 mM and MES 10 mM with better and worse growth, respectively. No growth is observed in the BM-7 medium without buffer. pH increase with all the buffers is similar or higher than the BM-7 control. a Cell growth represented as optical density, b Cell growth in Logarithmic (log2) scale of the optical density, and c pH of the external medium over time during cell growth