Fig. 13From: Growth parameters of Liberibacter crescens suggest ammonium and phosphate as essential molecules in the Liberibacter-plant host interfaceAccumulation of ammonia in M15 cell-free supernatant of L. crescens cultures over a 13-day period. L. crescens was grown to exponential phase in Hi-GI at 250 rpm, 28 °C (OD600~0.7–0.8, Fig. 16) and washed with a sterile ACES-KOH buffer pH 6.5 prior to inoculation. Concentration of ammonia/ammonium in the cell-free supernatant increased from 0.611 to 9.47 mM along with bacterial growth and pH increase of M15 mediumBack to article page