Fig. 1

Optimization of RPA reaction time. Genomic DNA of V. parahaemolyticus was amplified with RPA for different lengths of time, a clear DNA band with the expected size (168 bp) could be visualized by agarose gel electrophoresis after a 20 min reaction. Semi-quantification of the DNA band density using image Bio-1D software of VILBER Fusion FX5 automatic gel imaging instrument (Vilber, Marne La Vallée, France) revealed that no significant difference was observed in the product yields of 30 min and 40 min reactions. M, DNA marker, lanes 1–4, DNA products from reactions incubated for 10 min, 20 min, 30 min and 40 min