Fig. 2

The variable genomic locations of Tet P among isolates of P. sordellii. Shown are blastn alignments of P. sordellii isolates that encode Tet P or isolates with related loci. Regions of nucleotide identity between sequences are represented by grey bars; the higher the identity, the darker the grey, as illustrated by the legends. The cut off for nucleotide identity was a maximum e value of 0.001 with no minimum identity values over a length of 150 bases. Produced using EasyFig [43]. a P. sordellii isolates that encode Tet P on the chromosome in comparison to the related genomic location in isolates ATCC 9714 and W2922. ORFs are coloured according to known/predicted function (refer to key). b The novel P. sordellii tetracycline resistance plasmids pCSTC1 and pCSTC2. The sequence for pCSTC1 is from isolate R28058 but is also present in R15892 (see Fig. 2c). The sequence for pCSTC2 is from isolate SSCC37615 but is also found in isolates R32977 and SSCC18838 (see Fig. 2c). ORFs shown in light blue encode hypothetical proteins, ORFs shown in colour are annotated with their gene name or the predicted function of their product; tetracycline resistance genes tetA(P) (red) and tetB(P) (blue), putative DNA binding protein (DNAb) (orange), plasmid replication protein (Rep) (green) and conjugative relaxase (Mob) (brown). c Comparison of the Tet P containing plasmid sequences identified in P. sordellii isolates. ORFs are colored according to the gene they represent or the predicted function of their product (refer to key). ORFs truncated due to a break in the contig are marked with ‘*’