Table 3 Oligonucleotides used in this study
From: Characterization of HicAB toxin-antitoxin module of Sinorhizobium meliloti
Names | Sequences* | Restriction sites |
|---|---|---|
Tox pBAD-A | TGGGCTAGCGTGTGTATTGTCGTATCAGATG | NheI |
Tox pBAD-B | TGCCGTCGACTTACCTCAATTTCAAACC | SalI |
HicBNde | GAGGTAAGCATATGCGCAAC | NdeI |
HicBXho | GGTATCTCGAGCACATTTTTG | XhoI |
RHHNde | AGGTTCATATGTCCGACGCC | NdeI |
ToxXbaT | CATCTAGAGAGCGGCGAC | XbaI |
ToxEcoT | GAATTCCTCAATTTCAAACCG | EcoRI |
DopXbaT | CCTCTAGACAACTATATCGG | XbaI |
DopEcoT | AGGAATTCACATTTTTGCTGG | EcoRI |
RHHXba | AATCTAGAATGTCCGACGCCGAGAACAGG | XbaI |
COGEco | TAGAATTCCAACCTCAAGGGAGGAGGG | EcoRI |
RHHNde | CGGTGAAAACATATGCAAAAAGAG | NdeI |
HicBNH2Nde | GGCATATGCGCAACTATATCGGATTGATC | NdeI |
HicBNH2Xho | CCCTCGAGTTCGGCGAAGGCATCTATC | XhoI |
PET-Bgl | GGGGGGGAAGATCTAGAAATAATTTTGTTTAAC | BglII |
PET-EcoRI | CGGGAATTCAGCAAAAAACCCCTCAAG | EcoRI |
EMSAD | GATCCGACGGTTCGAGACCATCC | none |
EMSAR | TCTTCGGGTGAGGAACGGTAACC | none |