Fig. 3

Orthogonal images of RAW 264.7 macrophage CCL2-treated and non-treated, and infected by L. interrogans, allowing the observation of phagocytosed leptospires in different planes indicated in white squares. a and d) infected cells analyzed 1 h and 2 h post-infection, respectively; g and j) Cells CCL2-treated and infected, analyzed 1 h and 2 h post-infection respectively. White squares b, c, e, f, h, i, k and l on the images  are presented also amplified. m and n- percentage of pagocyted leptospires in the total of detected, by CCL2 non treated or treated cells respectively, in 1 h or 2 h after the infection. L. interrogans detected by antibodies FITC-conjugated. Nucleus of cells stained in blue by DAPI marker, cell’s cytoplasm stained in red by Evans blue. Leptospires were counted over five fields of each slide, being prepared three slides from each experimental treatment. Data are mean ± SD. Statistical differences and multiple comparison analysis were performed (*p < 0.05)