Putrescine biosynthesis and export genes are essential for normal growth of avian pathogenic Escherichia coli

Guerra, Priscila R.; Herrero-Fresno, Ana; Ladero, Victor; Redruello, Begoña; dos Santos, Teresa Pires; Spiegelhauer, Malene R.; Jelsbak, Lotte; Olsen, John Elmerdahl

doi:10.1186/s12866-018-1355-9

BMC Microbiology

Table 3 Strains and plasmids used in the study

From: Putrescine biosynthesis and export genes are essential for normal growth of avian pathogenic Escherichia coli

Strains and plasmids	Relevant features^a,b	Reference
E. coli strains
E. coli (WT-ST117)	Virulent reference strain, APEC strain	[5]
MG1655	MG1655/K-12- Reference strain	ATCC 2592
E. coli K-12 ER2420	Cloning intermediate strain	Termo Scientific
ΔspeB-ST117	speB mutant, Chl^R	This work
ΔspeC-ST117	speC mutant, Chl^R	This work
ΔspeB/C-ST117	speB mutant, speC mutant, Chl^R, Tmp^R	This work
ΔspeF-ST117	speF mutant, Chl^R	This work
ΔspeD/E-ST117	speD mutant, speE mutant, Chl^R	This work
ΔpotABCD-ST117	potABCD mutant, Chl^R	This work
ΔpotFGHI-ST117	potFGHI mutant, Chl^R	This work
ΔpotE-ST117	potE mutant, Chl^R	This work
ΔpotE/pACYC184	potE mutant, Chl ^R/ potE gene, Tet^R	This work
ΔyeeF-ST117	yeeF mutant, Chl^R	This work
ΔspeB-MG1655	speB mutant, Chl^R	This work
ΔspeC-MG1655	speC mutant, Chl^R	This work
ΔspeB/C-MG1655	speB mutant, speC mutant, Chl^R, Tmp^R	This work
ΔspeF-MG1655	speF mutant, Chl^R	This work
ΔspeD/E-MG1655	speD mutant, speE mutant, Chl^R	This work
ΔpotABCD -MG1655	potABCD mutant, Chl^R	This work
ΔpotFGHI -MG1655	potFGHI mutant, Chl^R	This work
ΔpotE -MG1655	potE mutant, Chl^R	This work
ΔpotF-MG1655	potE mutant, Chl^R	This work
Plasmids
pKD46	Plasmid with λ-Red recombinase expressed from arabinose inducible promoter	[39]
pKD3	Template plasmid for λ -red mutagenesis, Chl^R, Amp^R	Termo Scientific
pJET 1.2	Cloning vector, Amp^R	Termo Scientific
pACYC184	Expressing the ΔpotE gene, Chl^R, Tet ^R	Termo Scientific
pM3224T	Template plasmid for Tmp^R	[44]

^aThe metabolic pathway affected for each mutant is indicated in Fig. 1
^bChl^R chloramphenicol resistant; Tet^R tetracycline resistant; Gen^R gentamicin resistant; Tmp^R trimethoprim resistant; Amp^R ampicillin resistant

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ISSN: 1471-2180

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