Molecular characterization of Mannheimia haemolytica isolates associated with pneumonic cases of sheep in selected areas of Central Ethiopia

Table 1 Primer pairs flanking capsular genes of P. multocida and virulence associated genes of M. haemolytica used in the current study

Gene locus	Primer sequence (5′- 3′)		Size (bp)	Reference
CapA	Forward	5’-TGCCAAAATCGCAGTCAG- 3’	1044	[32]
CapA	Reverse	5′ -TTGCCATCATTGTCAGTG- 3’	1044	[32]
PHSSA	Forward	5′ -TTC ACA TCT TCA TCC TC-3’	325	[31]
PHSSA	Reverse	5′ TTT TCA TCC TCT TCG TC-3’	325	[31]
Rpt2	Forward	5′ - GTT TGT AAG ATA TCC CAT TT- 3’	1022	[31]
Rpt2	Reverse	5′- CGT TTT CCA CTT GCG TGA − 3’	1022	[31]

ISSN: 1471-2180