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Table 3 Analysis of PCR product lengths in nucleotides, single nucleotide polymorphisms (SNPs), insertion and deletions detected in the GpSGHV haplotypes in the Glossina species

From: Hytrosavirus genetic diversity and eco-regional spread in Glossina species

Haplotype

Species

Location

PCR Length: (SNPs, insertion, deletion) (bp)

No. of nonsynonymous mutations (aa)

VNTR-1

VNTR-2

SGHV009

SGHV010

SGHV038

(SGHV009, SGHV010, SGHV038)

GpSGHV-Uga

Reference

NCBI

370

437

535

1425

1461

Reference

H1

G. pallidipes

Tororo

370: (0,0,0)

437: (0,0,0)

535: (0,0,0)

1425: (0,0,0)

1461: (0,0,0)

(0,0,0)

G. m. morsitans

Ruckomechi

G. m. morsitans

Mfuwe

G. m. morsitans

Makuti

G. pallidipes

Ruckomechi

G. pallidipes

Mashumbi

G. m. morsitans

BioRI-KALRO

G. m. morsitans

Kwekivu

G. f. fuscipes

Buvuma Island

G. p. palpalis

Malanga

G. brevipalpis

Zululand

G. austeni

Zululand

H2

G. pallidipes

Tororo

370: (0,0,0)

500: (2,63,0)

535: (0,0,0)

1425: (0,0,0)

1461: (0,0,0)

(0,0,0)

H3

G. pallidipes

Tororo

370: (0,0,0)

500: (0,63,0)

535: (0,0,0)

1425: (0,0,0)

1461: (0,0,0)

(0,0,0)

H4

G. pallidipes

Tororo

370: (0,0,0)

374: (0,0,63)

535: (0,0,0)

1425: (0,0,0)

1461: (0,0,0)

(0.0,0)

G. m. morsitans

Mfuwe

G. austeni

Zululand

H5

G. pallidipes

Kwekivu

416: (12,66,20)

542: (15,105,0)

538: (1,3,0)

1425: (4,0,0)

1467: (12,6,0)

(1,1,4)

G. m. morsitans

Kwekivu

G. m. morsitans

Ruckomechi

G. m. morsitans

Mfuwe

H6

G. pallidipes

Kwamume

415: (13,65,20)

458: (14,21,1)

?

?

?

?

H7

G. pallidipes

Arba Minch

272: (8,1,99)

542: (24,126,20)

571: (14,36,0)

1425: (16,0,0)

1458: (26,0,3)

(19,12,10)

G. pallidipes

Ruckomechi

H8

G. pallidipes

Gokwe

?

483: (47,46,0)

?

1425: (14,0,0)

1460: (14,0,0)

(?,9,2)

G. pallidipes

Ruma N. Park

H9

G. pallidipes

Mwea

431: (11,61,0)

521: (30,84,0)

523: (7,0,12)

1425: (11,0,0)

1460: (20,0,1)

(3,7,5)

H10

G. pallidipes

Koibos Soi

?

458: (40,21,0)

529: (6,3,9)

1425: (15,0,0)

1461: (21,0,0)

(5,12,9)

H11

G. pallidipes

Koibos Soi

?

416: (36,0,21)

529: (6,3,9)

1425: (15,0,0)

1461: (21,0,0)

(5,12,9)

G. pallidipes

Emsos

H12

G. pallidipes

Emsos

?

416: (35,0,63)

529: (6,3,9)

1425: (15,0,0)

1461: (21,0,0)

(5,12,9)

H13

G. pallidipes

Mfuwe

?

374: (3,0,63)

535: (0,0,0)

1425: (0,0,0)

1461: (0,0,0)

(0,0,0)

G. m. morsitans

Mfuwe

H14

G. swynnertoni

Ikorongo GR

?

503: (36,67,0)

532: (3,0,3)

1425: (14,0,0)

1467: (16,6,0)

(1,9,6)

H15

G. pallidipes

Mwea

431: (11,61,0)

521: (30,84,0)

532: (3,3,6)

1425: (11,0,0)

1460: (20,0,1)

(2,7,5)

  1. The polymorphisms were based on the partial sequences of GpSGHV ORFs SGHV009, SGHV010, SGHV038, VNTR-1 and VNTR-2. The 3 numbers in brackets for each haplotype in column four to column eight refer to the number of SNPs, insertions and deletions respectively, relative to the GpSGHV-Uga reference sequences. The samples marked with a question mark (?) were not successfully sequenced. The nonsynonymous mutations of SGHV009, SGHV010, SGHV038 found in each sample are also shown in the last column