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Table 1 Bacterial strains and plasmids used in this work

From: RpfC regulates the expression of the key regulator hrpX of the hrp/T3SS system in Xanthomonas campestris pv. campestris

Strains or plasmids

Relevant characteristicsa

Source

X. c. pv. campestris

 8004

Wild type; Rifr

[24]

 XB001

8004/pL6hrpXsacB with a Tn5 insertion in XC_4007; Rifr; Kanr; Tcr

This work

 XB002

8004/pL6hrpXsacB with a Tn5 insertion in the intergenetic region

This work

 

between the ORFs XC_1510 and XC_1511; Rifr; Kanr; Tcr

 

 XB003

8004/pL6hrpXsacB with a Tn5 insertion in XC_2333; Rifr; Kanr; Tcr

This work

 XB004

8004/pL6hrpXsacB with a Tn5 insertion in XC_1192; Rifr; Kanr; Tcr

This work

 XB005

8004/pL6hrpXsacB with a Tn5 insertion in XC_3951; Rifr; Kanr; Tcr

This work

 XB006

8004/pL6hrpXsacB with a Tn5 insertion in XC_0124; Rifr; Kanr; Tcr

This work

 8004/pL6hrpXsacB

8004 harboring plasmid pL6hrpXsacB; Rifr; Tcr

This work

 ΔrpfC

rpfC in frame deletion mutant of 8004; Rifr

This work

 CΔrpfC

ΔrpfC harboring plasmid pLCrpfC; Rifr; Tcr

This work

 ΔrpfG

rpfG in frame deletion mutant of 8004; Rifr

[17]

 ΔavrBs1

avrBs1 in frame deletion mutant of 8004; Rifr; Gmr

[44]

 8004/pGUShrpG

8004 harboring plasmid pGUShrpG; Rifr; Tcr

This work

 ΔrpfC/pGUShrpG

ΔrpfC harboring plasmid pGUShrpG; Rifr; Tcr

This work

 8004/pGUShrpX

8004 harboring plasmid pGUShrpX; Rifr; Tcr

This work

 ΔrpfC/pGUShrpX

ΔrpfC harboring plasmid pGUShrpX; Rifr; Tcr

This work

E. coli

 JM109

RecA1, endA1, gyrA96, thi, supE44, relA1 Δ(lac-proAB)/F′ [traD36, lacIq, lacZ ΔM15]

[29]

Plasmids

 pUC19

Cloning vector; Ampr

[28]

 pLAFR6

Broad host range IncP cloning cosmid; Tcr

[28]

 pK18mobsacB

Suicide plasmid in Xcc; Mob+ Tra−; Kanr

[27]

 pLGUS

pLAFR6 containing a 1832-bp gusA ORF (excluding ATG), Tcr

[31]

 pL6sacB

pLAFR6 containing a 1419-bp sacB gene, Tcr

This work

 pKrpfCsacB

pK18mobsacB containing the two flanking fragments of rpfC; Kanr

This work

 pUCPhrpG

pUC19 containing hrpG promoter; Ampr

This work

 pUCPhrpX

pUC19 containing hrpX promoter; Ampr

This work

 pGUShrpG

pLAFR6 containing hrpG promoter in frame fused with gus gene; Tcr

This work

 pGUShrpX

pLAFR6 containing hrpX promoter in frame fused with gus gene; Tcr

This work

 pL6hrpXsacB

pLAFR6 containing hrpX promoter in frame fused with sacB gene; Tcr

This work

 pLCrpfC

pLAFR6 containing the sequenced whole ORF of rpfC; Tcr

This work

  1. aAmpr, ampicillin-resistant; Gmr, gentamicin-resistant; Kanr, kanamycin-resistant; Rifr, rifampicin-resistant; Tcr, tetracycline-resistant