Strains or plasmids | Relevant characteristicsa | Source |
---|---|---|
X. c. pv. campestris | ||
 8004 | Wild type; Rifr | [24] |
 XB001 | 8004/pL6hrpXsacB with a Tn5 insertion in XC_4007; Rifr; Kanr; Tcr | This work |
 XB002 | 8004/pL6hrpXsacB with a Tn5 insertion in the intergenetic region | This work |
 | between the ORFs XC_1510 and XC_1511; Rifr; Kanr; Tcr |  |
 XB003 | 8004/pL6hrpXsacB with a Tn5 insertion in XC_2333; Rifr; Kanr; Tcr | This work |
 XB004 | 8004/pL6hrpXsacB with a Tn5 insertion in XC_1192; Rifr; Kanr; Tcr | This work |
 XB005 | 8004/pL6hrpXsacB with a Tn5 insertion in XC_3951; Rifr; Kanr; Tcr | This work |
 XB006 | 8004/pL6hrpXsacB with a Tn5 insertion in XC_0124; Rifr; Kanr; Tcr | This work |
 8004/pL6hrpXsacB | 8004 harboring plasmid pL6hrpXsacB; Rifr; Tcr | This work |
 ΔrpfC | rpfC in frame deletion mutant of 8004; Rifr | This work |
 CΔrpfC | ΔrpfC harboring plasmid pLCrpfC; Rifr; Tcr | This work |
 ΔrpfG | rpfG in frame deletion mutant of 8004; Rifr | [17] |
 ΔavrBs1 | avrBs1 in frame deletion mutant of 8004; Rifr; Gmr | [44] |
 8004/pGUShrpG | 8004 harboring plasmid pGUShrpG; Rifr; Tcr | This work |
 ΔrpfC/pGUShrpG | ΔrpfC harboring plasmid pGUShrpG; Rifr; Tcr | This work |
 8004/pGUShrpX | 8004 harboring plasmid pGUShrpX; Rifr; Tcr | This work |
 ΔrpfC/pGUShrpX | ΔrpfC harboring plasmid pGUShrpX; Rifr; Tcr | This work |
E. coli | ||
 JM109 | RecA1, endA1, gyrA96, thi, supE44, relA1 Δ(lac-proAB)/F′ [traD36, lacIq, lacZ ΔM15] | [29] |
Plasmids | ||
 pUC19 | Cloning vector; Ampr | [28] |
 pLAFR6 | Broad host range IncP cloning cosmid; Tcr | [28] |
 pK18mobsacB | Suicide plasmid in Xcc; Mob+ Tra−; Kanr | [27] |
 pLGUS | pLAFR6 containing a 1832-bp gusA ORF (excluding ATG), Tcr | [31] |
 pL6sacB | pLAFR6 containing a 1419-bp sacB gene, Tcr | This work |
 pKrpfCsacB | pK18mobsacB containing the two flanking fragments of rpfC; Kanr | This work |
 pUCPhrpG | pUC19 containing hrpG promoter; Ampr | This work |
 pUCPhrpX | pUC19 containing hrpX promoter; Ampr | This work |
 pGUShrpG | pLAFR6 containing hrpG promoter in frame fused with gus gene; Tcr | This work |
 pGUShrpX | pLAFR6 containing hrpX promoter in frame fused with gus gene; Tcr | This work |
 pL6hrpXsacB | pLAFR6 containing hrpX promoter in frame fused with sacB gene; Tcr | This work |
 pLCrpfC | pLAFR6 containing the sequenced whole ORF of rpfC; Tcr | This work |