Fig. 1

Flagellated but non-motile mutant of L. agilis. The nucleotide/amino acid sequences of the WT and mutated motB gene of L. agilis (a). Underlines represent the targeted codon/translation of the point mutation. Motility of L. agilis strains which have either WT or mutant motB gene (b). Overnight cultures of L. agilis BKN88 (WT) and BKN134 motB (D23A) in MRS-soft agar medium. Observation of flagella by electron microscopy (c). Flagellar filaments of BKN88 and BKN134 were visualized by negative staining