Fig. 3
From: msaABCR operon is involved in persister cell formation in Staphylococcus aureus
Persister cells in exponential growth phase. Strains USA300 LAC, msaABCR mutant and the complementation were grown to exponential growth phase. Strains were then exposed to high concentration of antibiotics: a. no antibiotic control, b. daptomycin (10 μg/ml, 10× MIC), c. vancomycin (25 μg/ml, 40× MIC), d. linezolid (50 μg/ml, 10× MIC), e. gentamicin (20 μg/ml, 4× MIC), and f. rifampicin (2.4 μg/ml, 40× MIC). Time 0 h represents antibiotic exposure point. These results represent the means of three independent experiments. Error bars represent SE. Student’s t-test (OriginPro) was used to compare the results of the wild-type to mutants at respective designated time points (* p-value <0.05, ** p-value <0.005)