Fig. 5From: A rapid seamless method for gene knockout in Pseudomonas aeruginosa Colony PCR of merodiploids. Lane 1, 400 ng 1 kB DNA ladder; lane 2, 10 μl of PCR product of a colony in which plasmid integrated via the downstream homologous sequence; lane 3, 10 μl of PCR product of a colony in which plasmid integrated via the upstream homologous sequenceBack to article page