A comparison of sequencing platforms and bioinformatics pipelines for compositional analysis of the gut microbiome

Table 1 Primer sequence information for all platforms

Platform	Primer Name	Sequence (5′ -3′)	Targeting Region
Roche 454	Roche Titanium Fusion Primer A	CCATCTCATCCCTGCGTGTCTCCGACTCAG	V1-V2
	Universal Bacterial Primer 8F	AGAGTTTGATCCTGGCTCAG	V1-V2
	Roche Titanium Primer B	CCTATCCCCTGTGTGCCTTGGCAGTCTCAG	V1-V2
	Reverse Bacterial Primer 338R	GCTGCCTCCCGTAGGAGT	V1-V2
Illumina MiSeq	Forward Primer	TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAGAGTTTGATCCTGGCTCAG	V1-V2
Illumina MiSeq	Reverse Primer	GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGCTGCCTCCCGTAGGAGT	V1-V2
Ion Torrent PGM	Forward Primer composed of Ion Torrent adapter A	CCATCTCATCCCTGCGTGTCTCCGACTCAG	V1-V2
	Universal Bacterial Primer 8F	AGAGTTTGATCCTGGCTCAG	V1-V2
	Reverse Primer of Ion Torrent trP1 adapter	CCTCTCTATGGGCAGTCGGTGAT	V1-V2
	Reverse Bacterial Primer 338R	GCTGCCTCCCGTAGGAGT	V1-V2

ISSN: 1471-2180