Table 2 Kinetic parameters of HisN and Cg0911 at 30 °C and pH 7.35, n = 6

k_cat [s⁻¹]

1.04 ± 0.06

0.013 ± 0.002

for HolP

K_m [μM]

23.6 ± 1.4

638.3 ± 70.5

k_cat/K_m [s⁻¹ M⁻¹]

4.41 × 10⁴

1.98 × 10¹

Hill coefficient

1.47 ± 0.18

1.83 ± 0.13

for Mg²⁺

K_m [μM]

644.8 ± 22.4

5155.8 ± 117.9

k_cat/K_m [s⁻¹ M⁻¹]

1.61 × 10³

2.46 × 10⁰

Hill coefficient

2.44 ± 0.20

3.04 ± 0.12