Fig. 5
From: Contribution of pilus type 2b to invasive disease caused by a Streptococcus agalactiae ST-17 strain
Wild-type COH1 and its pilus 2b mutant strain show equivalent phagocytosis and intracellular survival in murine macrophages. Monolayers of J774A.1 murine macrophages were incubated for 2 h with wild-type GBS COH1 or its Δbp-2b mutant (MOI 10:1). After infection, cultures were incubated in presence of antibiotics for up to 48 h. At time points 0, 2 h, 6 h, 24 h and 48 h cells were lysed and plated for bacterial quantification. Data are presented as the mean CFU +/− SD from two independent experiments performed in quadruplicate. No statistical differences between strains were observed (Mann-Withney test)