Fig. 3

Bacterial burdens and TNF-α levels are different in the organs of BALB/c mice infected with Bp 1026b or Bp 576a. a Bacterial CFU/ml of blood and bacterial CFU/organ, i.e. (b), Lung; (c), Liver; (d), Spleen. Statistical significance determined by Mann-Whitney statistical testing of the medians of the two groups. ** = p < 0.01 E), TNF-α levels in lung homogenate from 1026b are not significantly (ns) different than uninfected mice while TNF-α levels from 576a treated lungs were significantly different than untreated mice lungs by the One-way ANOVA test for comparing the three groups. ** = p < 0.01. Black circles indicate 1026b–infected mice. White squares indicate 576a–infected mice. White triangles indicate uninfected mice. f BALB/c survival proportions following intranasal challenge with 5000 CFU of each strain indicated. The black arrow indicates the 24 h time point for removal of organs during the pre-determined endpoint study in (a-e), the survival data is also presented in Fig. 7 for clarity. ns = not significant by the Log-rank test of survival curves