Addition of plant-growth-promoting Bacillus subtilis PTS-394 on tomato rhizosphere has no durable impact on composition of root microbiome

Table 3 Relative abundance of core kingdoms/phylum present in rhizosphere eukaryota community determined by ITS sequencing

Core Kingdom	Relative abundance (%) in Control treatment					Relative abundance (%) in PTS-394 treatment
Core Kingdom	1 d	3 d	7 d	9 d	14 d	1 d	3 d	7 d	9 d	14 d
Fungi	16.24	20.54	5.64	36.52	1.11	17.22	12.10	5.91	9.33	1.53
Viridiplantae	29.79	24.93	81.81	39.59	97.76	58.22	79.23	92.29	81.40	97.37
Metazoa	0.39	0.62	0.05	0.98	0.17	0.63	0.15	0.43	1.09	0.14
no_rank_Eukaryota group	20.37	21.83	6.21	20.69	0.20	22.27	7.93	1.08	7.04	0.94
unclassified_Eukaryota group	0.18	0.20	0.08	0.16	0.00	0.06	0.04	0.03	0.01	0.01
Unclassified group	33.03	31.87	6.21	2.05	0.76	1.59	0.55	0.27	1.12	0.00
Core phylum
Streptophyta	19.87	17.29	74.15	30.06	97.43	18.17	64.02	90.46	70.60	97.32
Chlorophyta	9.91	7.59	7.64	9.39	0.33	40.04	15.20	1.83	10.80	0.05
Ascomycota	2.17	3.98	1.30	8.42	0.13	2.26	1.44	0.59	0.67	0.63
Basidiomycota	1.94	1.83	0.83	2.10	0.17	1.60	4.36	0.94	1.13	0.16
Porifera	0.37	0.49	0.05	0.79	0.06	0.19	0.12	0.42	0.78	0.01
Chytridiomycota	0.18	0.42	0.20	0.58	0.03	0.40	0.19	0.11	0.23	0.07
Nematoda	0.03	0.09	0.00	0.15	0.12	0.41	0.03	0.01	0.11	0.10
no_rank_Eukaryota group	20.37	21.82	6.19	20.68	0.20	22.26	7.93	1.05	7.04	0.94
no_rank_Fungi group	11.19	12.94	3.13	18.86	0.67	12.44	5.75	4.18	6.99	0.66
unclassified_Eukaryota group	0.18	0.20	0.08	0.16	0.00	0.06	0.04	0.03	0.01	0.01
unclassified_Fungi group	0.71	1.30	0.18	6.48	0.10	0.44	0.28	0.08	0.14	0.01

The assay was performed 1, 3, 7, 9, and 14 days after inoculation with PTS-394, Control experiments without PTS-394 were performed at same time points

ISSN: 1471-2180