Fig. 1From: Cytotoxic Necrotizing Factor-1 (CNF1) does not promote E. coli infection in a murine model of ascending pyelonephritisConstruction of U8 cnf1 deletion mutant. a One-stp PCR deletion of cnf1 in U8 bacteria (Δcnf1). A chloramphenicol resistance cassette (CAT) was PCR-amplified from pKD3 with homologous extensions (H1 and H2) of the CNF1 locus and transferred to pKD46 Red recombinase-expressing U8 clones and selected for chloramphenicol resistance. b Western blot of U8, U8 cnf1 deletion mutant (Δcnf1), RS218, and RS218 cnf1 deletion mutants (R Δcnf1) with monoclonal CNF1 antibodyBack to article page