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Table 3 PCR Efficiency

From: Simultaneous detection of chikungunya virus, dengue virus and human pathogenic Leptospira genomes using a multiplex TaqMan® assay

 

Simplex Assay

Multiplex Assay

CHIKV

DENV

Leptospira

CHIKV

DENV

Leptospira

Slope

−3.13

−3.19

−3.25

−3.41

−3.15

−3.37

Efficiency

2.09

2.06

2.03

1.96

2.08

1.98

  1. Efficiency = 10(−1/slope)-1