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Fig. 2 | BMC Microbiology

Fig. 2

From: Transposon insertion sequencing reveals T4SS as the major genetic trait for conjugation transfer of multi-drug resistance pEIB202 from Edwardsiella

Fig. 2

Transfer frequency of pEIB202 under different conditions. The WT strain (donor, Strr/Colr) carrying pEIB202 plasmid was conjugated with the EIB202 plasmid-cured strain with Km resistant gene inserted in a neutral site of the chromosome (EIB202 ∆P::pNQ705K) (recipient, Kmr/Colr) and the conjugants were selected on Str/Km/Col LB agar plates. a Strains were conjugated for different time under 30 °C. b Strains were conjugated under different temperatures for 8 h. c and d Strains were conjugated at different antibiotics at their sublethal concentrations (1/4 of MIC) and culture conditions under 30 °C for 8 h. e The transfer frequency between E. piscicida and other bacterial species. Results were presented as mean ± SD (n = 3). * P < 0.01, ** P < 0.001, ***P < 0.0001, student’s t-test

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BMC Microbiology

ISSN: 1471-2180

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