Fig. 4
From: Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers
Confocal laser scanning microscopy of frozen section of nasal tissue from an S. aureus carrier. Overview (a-b) and detailed/zoomed portion of image (c-d) showing S. aureus in stratum spinosum. Scale bar is in micrometers. Green fluorescence labeling of S. aureus, blue fluorescence labeling of keratinocyte cell nuclei and S. aureus DNA, red fluorescence labeling of actin. Primary antibody rabbit polyclonal antibody to S. aureus (ab20920, Abcam), secondary antibody Alexa Fluor 488® goat anti-rabbit IgG (Molecular Probe™, Thermo Fisher Scientific), Alexa Fluor 594 Phalloidin (Molecular Probes™, Thermo Fisher Scientific) and DRAQ5 (BioStatus). We used a CLSM ×63 objective