Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study

Table 2 Limit of detection of HRM assay

Parameter	Genus	Species ^a	Cluster ^b	gDNA amount (pg)
Parameter	Genus	Species ^a	Cluster ^b	1000	500	50	10	5	1	0.5	0.1	0.05	0.01
Detection ^c	Aspergillus	creber	3	12/12	12/12	12/12	12/12	12/12	12/12	11/12	6/12	0/12	0/12
	Aspergillus	sydowii	2	12/12	12/12	12/12	12/12	12/12	12/12	11/12	9/12	0/12	0/12
	Aspergillus	versicolor	1	12/12	12/12	12/12	12/12	12/12	12/12	12/12	12/12	10/12	0/12
Confidence (%) ^d	Aspergillus	creber	3	97.98 ± 1.28	96.66 ± 1.54	99.03 ± 0.59	99.30 ± 0.29	99.70 ± 0.02	97.99 ± 0.53	96.65 ± 0.36	97.99 ± 0.53.	/	/
	Aspergillus	sydowii	2	98.72 ± 0.39	99.25 ± 0.26	99.28 ± 1.66	99.67 ± 1.12	98.840 ± 2.34	96.83 ± 3.11	95.63 ± 1.63	95.83 ± 3.11	/	/
	Aspergillus	versicolor	1	98.99 ± 0.26	99.23 ± 0.35	99.49 ± 2.01	98.93 ± 0.60	99.76 ± 096	98.52 ± 2.32	97.49 ± 0.11	97.60 ± 1.06	96.37 ± 1.540	/

^agDNA extracted from pure strains from the BCCM/IHEM collection. BCCM/IHEM strains used as reference: A. creber IHEM 2646, A. sydowii IHEM 26347, A. versicolor IHEM 18884
^bCluster defined with the Biorad Precision Melt Analysis software 1.2 (Temse, Belgium)
^cDetection: Number of positive signals i.e., correct cluster and confidence >95%, observed for each dilution, the LOD for each species is indicated in bold
^dConfidence (%) is the average and standard deviation (±SD)

ISSN: 1471-2180