Table 3 Zeocin-induced mutagenesis in Δpnp strains
Strain | Chrom. alleles | Plasmid allele | Fraction surviving (±SD) | Spontaneous mutants (×109) | Zeocin-induced mutants (×109) | Fold induction | Ratio to pnp + |
|---|---|---|---|---|---|---|---|
C-1a/pGZ119HE | wild | none | 0.40 (±0.014) | 273.7 (±44.6) | 1116.3 (±125) | 4.08 | 0.95 |
C-5691/pAZ101 | Δpnp | pnp + | 0.37 (±0.028) | 77.4 (±19.6) | 331.4 (±82.8) | 4.28 | 1.00 |
C-5691/pGZ119HE | Δpnp | none | 0.38 (±0.015) | 278.2 (±70.8) | 530.7 (±98.0) | 1.91 | 0.45 |
C-5691/pAZ1112 | Δpnp | pnpS438A | 0.40 (±0.026) | 123.9 (±55.2) | 222.4 (±85.2) | 1.80 | 0.42 |
C-5691/pAZ1113 | Δpnp | pnp-ΔKH | 0.31 (±0.073) | 15.8 (±11.7) | 108.0 (±75.9) | 6.81 | 1.59 |
C-5691/pAZ1114 | Δpnp | pnp-ΔS1 | 0.39 (±0.035) | 16.7 (±7.8) | 119.0 (±55.4) | 7.14 | 1.67 |
C-5691/pAZ133 | Δpnp | pnp-ΔKHS1 | 0.33 (±0.035) | 49.6 (±14.2) | 208.4 (77.3) | 4.20 | 0.98 |
C-5691/pAZ1115 | Δpnp | rnb + | 0.41 (±0.023) | 97.4 (±15.9) | 189.3 (±16.2) | 1.94 | 0.45 |
C-5981/pAZ1115 | Δrnb | rnb + | 0.39 (±0.029) | 48.6 (±23.8) | 204.0 (±85.1) | 4.20 | 0.98 |
C-5981/pGZ119HE | Δrnb | none | 0.36 (±0.027) | 228.7 (±50.8) | 992.5 (±222.8) | 4.34 | 1.01 |
C-5981/pAZ101 | Δrnb | pnp + | 0.38 (±0.019) | 153.9 (±38.1) | 638.4 (±159.4) | 4.15 | 0.97 |