The proteins of Fusobacterium spp. involved in hydrogen sulfide production from L-cysteine

BMC Microbiology

Table 1 Bacteria examined for hydrogen sulfide (H₂S)-producing enzymes, identified with in-gel cysteine digestion and bismuth staining^a

Species	Subspecies	Strain	Broth
Fusobacterium canifelinum		CCUG^b 66382	Todd Hewitt
Fusobacterium necrophorum	funduliforme	ATCC^c 51357	Todd Hewitt
Fusobacterium necrophorum		CCUG 48192	Todd Hewitt
Fusobacterium nucleatum	polymorphum	ATCC 10953	Todd Hewitt
Fusobacterium nucleatum		OMGS^d 3938^e	Todd Hewitt
Fusobacterium periodonticum		ATCC 33693	Todd Hewitt
Fusobacterium periodonticum		CCUG 66383	Todd Hewitt
Parvimonas micra		ATCC 33270	BHI^f + 10% serum
Porphyromonas endodontalis		OMGS 1205	BHI + 10% serum
Porphyromonas gingivalis (W83)		OMGS 197	BHI
Porphyromonas gingivalis (381 F)		CCUG 14449	BHI
Prevotella intermedia		ATCC 25611	BHI
Prevotella tannerae		ATCC 51259	BHI
Treponema denticola		OMGS 3271^g	Spirochete broth^h

^aBacterial species were grown in broth until OD₆₀₀ of approximately 0.8. After washing and centrifugation, the cells were lysed and the proteins were separated in gel by molecular weight, before staining in bismuth(III)chloride solution containing cysteine. The cysteine-degrading proteins that produced H₂S were identified in the assay by color change; Sulfide from H₂S reacted with bismuth and formed bismuth sulfide, a black precipitate. Another set of gels were also stained with conventional Coomassie staining. All experiments were repeated at least once
^bCulture Collection University of Gothenburg
^cAmerican Type Culture Collection
^dOral Microbiology Gothenburg Sweden
^eOriginally received from Malmö (Badersten 5U)
^fBrain Heart Infusion broth with 2 mL/L menadione and 10 mL/L hemin
^gOriginally received from Dr R. Ellen, University of Toronto, Toronto, Canada
^hDawson JR, Ellen RP. Tip-oriented adherence to Treponema denticola to fibronectin. Infect Immun. 1990//;58(12):3924–8

ISSN: 1471-2180