Fig. 2From: Zinc stress induces copper depletion in Acinetobacter baumannii Transcriptional analyses of putative A. baumannii Zn and Cu efflux systems. The mRNA transcription levels of the putative (a) Cation Diffusion Facilitator (CDF), (b) Heavy Metal Efflux (HME), (c) P-type ATPase, (d) CorA-type and (e) CopB-like copper resistance genes of A. baumannii strain ATCC 17978 were determined by qRT-PCR. Transcription levels were examined in the presence of 400 μM Zn, 400 μM Cu or 400 μM Zn + 400 μM Cu and corrected to untreated cells following internal normalisation to GAPDH. The data are the mean of at least biological triplicates (± SEM). Statistical analyses were performed by one-way ANOVA using Dunnett’s posttest; ns = not significant, * = p < 0.05, ** = p < 0.01, *** = p < 0.001 and **** = p < 0.0001Back to article page