Table 1 Bacterial strains and plasmids used in this study

 DH5α

supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

Hanahan 1983 [47]

 BL21

For protein expression

Novagen

 PXO99^A

Wildtype strain, Philippine race 6

Lab collection

 ∆catB

catB gene deletion mutant derived from PXO99^A, Gm^r

This study

 ∆catB(pBBR-catB)

Complementary bacterium strain of ∆catB, Ap^r

This study

 ∆oxyR

oxyR gene deletion mutant derived from PXO99^A, Gm^r

Our lab

 ∆oxyR(pBBR-oxyR)

Complementary bacterium strain of ∆oxyR, Ap^r

Our lab

 pMD18-T

Cloning vector, Ap^r

TaKaRa, Tokyo

 pET-28a

Expression vector to generate a N-terminal His₆ tag, Km^r

Haigene

 pK18mobsacB

Suicidal vector carrying sacB gene for mutagenesis, Gm^r

Schafer et al., 1994 [44]

 pBBR1MCS-4

Broad-host range expression vector, Ap^r

Kovach et al., 1995 [48]

 pHM1

Broad-host range expression vector, Sp^r

Hopkins et al., 1992 [49]

 pHT304BZ

Promoterless lacZ vector, Ap^r

Lereclus et al., 1996 [50]

 pMDcatB

pMD18-T derivative carrying the full length of catB, Ap^r

This study

 pMDcatBr

pMD18-T derivative carrying the right fragment of catB, Ap^r

This study

 pMDcatBl

pMD18-T derivative carrying the left fragment of catB, Ap^r

This study

 pMDoxyR

pMD18-T derivative carrying the full length of oxyR, Ap^r

This study

 pKcatB

pK18mobsacB derivative carrying the full length of catB, Gm^r

This study

 pEToxyR

pET-28a derivative carrying the full length of oxyR, Km^r

This study

 pBBR-catB

pBBR1MCS-4 derivative carrying the full length of catB, Ap^r

This study

 pHTpB

pHT304BZ derivative carrying the promoter region of catB, Ap^r

This study

 pH-lacZ

pHM1 derivative carrying the promoterless lacZ, Sp^r

This study

 pH-catBp-lacZ

pHM1 derivative carrying the promoter region of catB and promoterless lacZ, Sp^r

This study